Abstract

Culex quinquefasciatus are among the most important vectors of arboviral diseases worldwide. Recent
studies indicate that diverse midgut microbiota of mosquitoes significantly affects development, digestion,
metabolism, and immunity of their hosts. Here, we studied the bacterial diversity found in midgut part
of C. quinquefasciatus to understand the host and microbe interaction. The adult C. quinquefasciatus
mosquitos were collected from Loyola College Campus, Chennai, using ovitraps, and midgut part was
extracted; moreover, the DNA templates were isolated and amplified by polymerase chain reaction. The
DNA amplicons were sequenced by Illumina MiSeq gene sequencer. The total of 279,157 reads was
classified into 85, the bacterial genera of Pseudomonas, Klebsiella, Staphylococcus, and Aeromonas
predominantly found to be high when compared to the other bacterial genera. The present data strongly
encourage further investigations to verify the potential role of the detected bacteria in mosquito for the
transmission of several vectoral diseases.