In the present study, five new derivatives (R1 to R5) of benzothiazoles were synthesized and were evaluated against four fungal strains: Candida albicans (MTCC 183), Aspergillus niger (MTCC 228), Candida tropicalis (MTCC 6192), and Fusarium oxysporium (MTCC 3656). p-Toluidine on treatment with ammonium thiocynate formed 2-benzothiazolamines, which on reacted with hydrazine hydrate formed a hydrazino derivative. Compounds (R1 to R5) were synthesized by reacting hydrazine derivative with different substituted acetophenones. All the synthesized compounds were identified by IR, 1H-NMR and antifungal activity was performed on the synthesized compounds. The results obtained justify the importance of benzothiazoles in the field of therapeutics