The present study describes the anti-bacterial activity of hydro-alcoholic extract of Terminalia chebula Retz fruit against microorganism like: Bacillus substils, Staphylococcus aureus, Staphylococcus epidermis, Escherichia coli, Staphylococcus flexineria and Pseudomonas aeruginosa. For this purpose hydro-alcoholic extract of fruit were prepared and tested by “Disc Diffusion Method”. As a result of this study it was found that the extract of fruit generally revealed anti-bacterial activity against both gram-positive bacteria (B. subtils, S. aureous and S.epidermis) and gram-negative bacteria (E. coli, S. flexineria and S. auriginosa).