Dental plaque represents a complex community of different species of microorganisms which include a majority of streptococci and other microorganisms. Samples collected from 19 healthy individuals of different age groups from rural population of Sagar, Madhya Pradesh, yielded 80 isolates of genus Streptococcus of 17 different species including Streptococcus agalactiae, S. cremoris, S. equinus, S. faecalis var malodoratus, S. faecium, Streptococcus grp F, Streptococcus grp H, Streptococcus grp O, Streptococcus grp Q1, S. lactis, S. milleri, S. mtior, S. mutans sub sp. sobrinus, S. pneumonae, S. pyogenes, S. sanguis I, S. uberis. Out of these, most dominating species was found to be S. faecium followed by S. milleri and S. cremoris. Sixty five isolates were found α-haemolytic while remaining isolates showed no haemolysis (γ-haemolytic).