Novel evaluation of In vitro Anti-inflammatory and Free Radical Scavenging activities of Ficus krishnae

Abstract

Ficus krishnae also known as “Makkhankathor†belongs to family Moraceae and is reported to be highly medicinal in India. As a folk medicine, the plant is used in treatment of broad range of diseases and disorders. In the present study the methanol and aqueous extracts of Ficus krishnae was evaluated for its total phenolic, Flavonoid content, in vitro anti-inflammatory and free radical scavenging activity of Ficus krishnae. Invitro anti-inflammatory activity by inhibition of protein denaturation method and antioxidant potential of extract was carried out by free radical scavenging activity using 2, 2 diphenyl 1-picryl hydrazyl (DPPH), ABTS radical scavenging activity, reducing power assay and phosphomolybdenum assay by using ascorbic acid and BHT as reference standards. Total phenol and flavonoid content of methanol extracts found to be 0.34mg/g and 0.60mg/g. whereas aqueous extract was to be 0.14mg/g and 0.10mg/g. Invitro anti-inflammatory study of polar solvent extracts has showed that the crude methanol extract of F.krishnae is a potential source of natural inhibition of protein denaturation and antioxidants this justified its uses in folkloric medicine.