Method Development and Validation of Sulphadiazine in Bulk and Pharmaceutical Dosage Form by UV-Spectrophotometric Method

Authors

  • Kothakota Vandana Department of Pharmaceutical Analysis and Quality Assurance, Royal College of Pharmacy and Health Sciences, Andhapasara Road, Berhampur, Odisha.

Abstract

ABSTRACT: A new, simple, precise and accurate method for the estimation of Sulphadiazine in bulk and pharmaceutical dosage form has been developed. A mixture of methanol and water (90:10) was chosen as the solvent system. The λmax was found to be 270nm. The responses were linear in the range of 05-30µg/ml. The regression equation of the calibration graph and correlation coefficient were found to be y = 0.099x + 0.031 and 0.999 respectively. Validation of the method was done in order to demonstrate accuracy, precision, interday and intraday assay, robustness and ruggedness of the proposed method. The %RSD values for both intraday and interday precision were less than 1%. The recovery of the drug from the sample was ranged between 99.48% and 100.90%. Commercial tablets containing 500mg of Sulphadiazine (SULPHADIAZINE manufactured by Abbot Healthcare Pvt. Ltd. Mumbai) were analyzed by the proposed method and the results were well within the claimed limits.

Downloads

Download data is not yet available.

Downloads

How to Cite

Vandana, K. (2011). Method Development and Validation of Sulphadiazine in Bulk and Pharmaceutical Dosage Form by UV-Spectrophotometric Method. International Journal of Pharmaceutical & Biological Archive, 2(4). Retrieved from http://ijpba.info/index.php/ijpba/article/view/352

Issue

Vol. 2 No. 4 (2011): IJPBA

Section

Research Articles

License

This is an Open Access article distributed under the terms of the Attribution-Noncommercial 4.0 International License [CC BY-NC 4.0], which requires that reusers give credit to the creator. It allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, for noncommercial purposes only.