Production of Extracellular Polysaccharides by a Rhizobium Species from Root Nodules of Vigna mungo (Hepper)

Authors

  • Dr. P Nirmala PG and Research Department of Microbiology, Mohamed Sathak College of Arts and Science, Sholinganallur, Chennai, Tamilnadu, India.

Abstract

ABSTRACT The ability of ten Rhizobium strains, isolated from root nodules of leguminous crop, Vigna mungo (L.) Hepper were tested for their production of Extracellular Polysaccharides (EPS) in Yeast Extract Mineral (YEM) medium. Among the ten strains, maximum amount of EPS was produced by Rhizobium Vm6 strain (1680 µg/mL). The maximum amount of EPS production by Rhizobium strain was achieved by optimizing the cultural conditions (Carbon and Nitrogen sources). The EPS production was maximum, when the medium was supplemented with mannitol (2%) and potassium nitrate (0.2%) which was accompanied by a great increase in the production compared to the control. The EPS contained glucose, galactose, xylose, rhamnose and raffinose, which were identified by paper chromatography.

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How to Cite

Nirmala, D. P. (2011). Production of Extracellular Polysaccharides by a Rhizobium Species from Root Nodules of Vigna mungo (Hepper). International Journal of Pharmaceutical & Biological Archive, 2(4). Retrieved from http://ijpba.info/index.php/ijpba/article/view/360

Issue

Vol. 2 No. 4 (2011): IJPBA

Section

Research Articles

License

This is an Open Access article distributed under the terms of the Attribution-Noncommercial 4.0 International License [CC BY-NC 4.0], which requires that reusers give credit to the creator. It allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, for noncommercial purposes only.